CPK isoenzymes test
The CPK isoenzymes test measures the different forms of creatine phosphokinase (CPK) in the blood. CPK is an enzyme found mainly in the heart, brain, and skeletal muscle.
Creatine phosphokinase - isoenzymes; Creatine kinase - isoenzymes; CK - isoenzymes
How the Test is Performed
A blood sample is needed. This may be taken from a vein. The test is called a venipuncture.
If you are in the hospital, this test may be repeated over 2 or 3 days. A significant rise or fall in the total CPK or CPK isoenzymes can help your health care provider diagnosis certain conditions.
How to Prepare for the Test
Usually, no special preparation is necessary.
Tell your doctor about all the medications you are taking. Certain medications can interfere with test results. Drugs that can increase CPK measurements include the following:
- Amphotericin B
- Certain anesthetics
- Fibrate drugs
- Steroids such as dexamethasone
This list is not all-inclusive.
How the Test Will Feel
When the needle is inserted to draw blood, you may feel moderate pain, or only a prick or stinging sensation. Afterward, there may be some throbbing.
Why the Test is Performed
This test is done if a CPK test reveals that your total CPK level is elevated. CPK isoenzyme testing can help pinpoint the exact source of the damaged tissue.
CPK is made of three slightly different substances:
- CPK-1 (also called CPK-BB) is found mostly in the brain and lungs
- CPK-2 (also called CPK-MB) is found mostly in the heart
- CPK-3 (also called CPK-MM) is found mostly in skeletal muscle
What Abnormal Results Mean
Higher-than-normal CPK-1 levels:
Because CPK-1 is found mostly in the brain and lungs, injury to either of these areas can increase CPK-1 levels. Increased CPK-1 levels may be due to:
Higher-than-normal CPK-2 levels:
CPK-2 levels rise 3 - 6 hours after a heart attack. If there is no further heart muscle damage, the level peaks at 12 - 24 hours and returns to normal 12 - 48 hours after tissue death.
Increased CPK-2 levels may also be due to:
- Electrical injuries
- Heart defibrillation (purposeful shocking of the heart by medical personnel)
- Heart injury (for instance, from a car accident)
- Inflammation of the heart muscle usually due to a virus (myocarditis)
- Open heart surgery
CPK-2 levels do NOT usually rise with chest pain caused by angina, pulmonary embolism (blood clot in the lung), or congestive heart failure.
Higher-than-normal CPK-3 levels are usually a sign of muscle injury or muscle stress and may be due to:
- Crush injuries
- Muscle damage due to drugs or being immobile for a long time (rhabdomyolysis)
- Muscular dystrophy
- Myositis (skeletal muscle inflammation)
- Receiving many intramuscular injections
- Recent nerve and muscle function testing (electromyography)
- Recent seizures
- Recent surgery
- Strenuous exercise
Factors that can affect test results include cardiac catheterization, intramuscular injections, recent surgery, and vigorous and prolonged exercise or immobilization.
Isoenzyme testing for specific conditions is about 90% accurate.
Anderson JL. ST segment elevation acute myocardial infarction and complications of myocardial infarction. In: Goldman L, Schafer AI, eds. Cecil Medicine. 24th ed. Philadelphia, Pa: Saunders Elsevier; 2011:chap 73.
Chinnery PF. Muscle diseases. In: Goldman L, Schafer AI, eds. Cecil Medicine. 24th ed. Philadelphia, Pa: Saunders Elsevier; 2011:chap 429.
David C. Dugdale, III, MD, Professor of Medicine, Division of General Medicine, Department of Medicine, University of Washington School of Medicine. Also reviewed by A.D.A.M. Health Solutions, Ebix, Inc., Editorial Team: David Zieve, MD, MHA, David R. Eltz, Stephanie Slon, and Nissi Wang.
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